Modified Oligonucleotides
1. PS, PO and small nucleic acids of mixed backbone
PS bonds can be introduced between the last 3-5 nucleotides of the 5'- or 3'-end of oligonucleotides to inhibit exonuclease degradation. Incorporating PS bonds throughout the oligonucleotides is also helpful in reducing endonuclease attacks.
2. Fluorescent labels, such as FAM, Cy5 and Cy3
Quantitative analysis and specific recognition of DNA molecules are of great significance to the development of genomics, virology, molecular biology and other related disciplines. However, due to weak fluorescence of biomolecules themselves, most DNA sequences are fluorescently labeled at present, and labeled DNA probes have been widely applied to automatic DNA sequencing, antibody immunoassay, disease diagnosis, anticancer drug analysis, and so forth.
Suzhou Olipharma provides a variety of common fluorescent modification groups, which are generally used as DNA probes and can be modified at the 5' end or 3' end:
|
3’end |
5’end |
|
BHQ 0 |
ROX |
|
BHQ 1 |
Cy3 |
|
BHQ 2 |
Cy5 |
|
Cy3 |
Biotin |
|
Cy5 |
FAM |
|
Chol |
VIC |
3. Oligonucleotides coupling, linker and ligand synthesis (GalNAc, PEG, peptide modifications, etc.)
Primary amine groups can be used to link various modification groups to oligonucleotides. Amino modifications can be placed at the 5' end, usually including C6 or C12; or amino modifications may be positioned at the 3' end, usually including C7, etc. Oligonucleotides linked to primary amines can be coupled with the remaining other types of molecules, so as to expand the application range of oligonucleotide drugs.
Suzhou Olipharma provides a variety of common oligonucleotides coupling products, which can be bound with SMCC, PEG-Azides, Peptide, etc., and also offers various GalNac modified oligonucleotides, such as L96, NAG37.
